Review



pbfv u6 2 vector  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Addgene inc pbfv u6 2 vector
    Pbfv U6 2 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbfv+u6+2/pBFv-U6%2E2+(Plasmid+%23138400)/bio_rxiv__2025__04__25__650632-170-31-33
    Average 93 stars, based on 10 article reviews
    pbfv u6 2 vector - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Pleiotropic fitness effects of the lncRNA Uhg4 in Drosophila melanogaster
    Article Snippet: .. One double-stranded gRNA was cloned into pBFv-U6.2 (Addgene #138400), and the other double-stranded gRNA was cloned into pBFv-U6.2B (Addgene #138401). ..

    Article Title: The scaffolding protein Cnk binds to the receptor tyrosine kinase Alk to promote visceral founder cell specification in Drosophila .
    Article Snippet: .. Target sequences were cloned into the pBFv-U6.2 or pU6-BbsI-chiRNA gRNA expression vectors (GEPC, respectively; Addgene). .. To achieve molecularly defined deletions, we forced homologous recombination by simultaneously injecting (BestGene Inc.) sgRNA(s) and the respective pBluescript gene editing donor construct into embryos carrying the P{FRT(whs)}G13 insertion and expressing Cas9 in their germ line (M{vas-Cas9}ZH-2A).

    Article Title: Knock-in mutagenesis in Drosophila Rdl underscores the critical role of the conserved M3 glycine in mediating the actions of broflanilide and isocycloseram on GABA receptors.
    Article Snippet: γ-Aminobutyric acid receptors (GABARs) are crucial targets for pest control chemicals, including meta-diamide and isoxazoline insecticides, which act as negative allosteric modulators of insect GABARs.. Previous cell-based assays have indicated that amino acid residues in the transmembrane cavity between adjacent subunits of Drosophila RDL GABAR (i.e., Ile276, Leu280, and Gly335) are involved in mediating the action of meta-diamides.. In this study, to confirm this result at the organismal level, we employed CRISPR/Cas9-mediated genome editing, generated six transgenic Drosophila strains carrying substitutions in these amino acid residues, and investigated their sensitivity to broflanilide and isocycloseram.

    Article Title: Pleiotropic fitness effects of the lncRNA Uhg4 in Drosophila melanogaster
    Article Snippet: .. One double-stranded gRNA was cloned into pBFv-U6.2 (Addgene #138,400), and the other double-stranded gRNA was cloned into pBFv-U6.2B (Addgene #138,401). ..

    Article Title: Pleiotropic fitness effects of the lncRNA Uhg4 in Drosophila melanogaster.
    Article Snippet: .. One double-stranded gRNA was cloned into pBFv-U6.2 (Addgene #138,400), and the other double-stranded gRNA was cloned into pBFv-U6.2B (Addgene #138,401). ..

    CRISPR:

    Article Title: A missense mutation in human INSC causes peripheral neuropathy.
    Article Snippet: .. The gRNA for CRISPR/Cas9 target sites was designed using UCSC genome browser (https://genome.ucsc.edu/), and ligated into pBFv-U6.2 (Addgene #138400) plasmid. .. To clone the donor DNA plasmid, both upstream and downstream homology arms of dInsc were amplified from fly genomic DNA by Phusion® High-Fidelity DNA Polymerase (New England Biolab #M0530S) under optimized conditions.

    Article Title: A missense mutation in human INSC causes peripheral neuropathy
    Article Snippet: .. The gRNA for CRISPR/Cas9 target sites was designed using UCSC genome browser ( https://genome.ucsc.edu/ ), and ligated into pBFv-U6.2 (Addgene #138400) plasmid. .. To clone the donor DNA plasmid, both upstream and downstream homology arms of dInsc were amplified from fly genomic DNA by Phusion® High-Fidelity DNA Polymerase (New England Biolab #M0530S) under optimized conditions.

    Plasmid Preparation:

    Article Title: A missense mutation in human INSC causes peripheral neuropathy.
    Article Snippet: .. The gRNA for CRISPR/Cas9 target sites was designed using UCSC genome browser (https://genome.ucsc.edu/), and ligated into pBFv-U6.2 (Addgene #138400) plasmid. .. To clone the donor DNA plasmid, both upstream and downstream homology arms of dInsc were amplified from fly genomic DNA by Phusion® High-Fidelity DNA Polymerase (New England Biolab #M0530S) under optimized conditions.

    Article Title: Knock-in mutagenesis in Drosophila Rdl underscores the critical role of the conserved M3 glycine in mediating the actions of broflanilide and isocycloseram on GABA receptors.
    Article Snippet: γ-Aminobutyric acid receptors (GABARs) are crucial targets for pest control chemicals, including meta-diamide and isoxazoline insecticides, which act as negative allosteric modulators of insect GABARs.. Previous cell-based assays have indicated that amino acid residues in the transmembrane cavity between adjacent subunits of Drosophila RDL GABAR (i.e., Ile276, Leu280, and Gly335) are involved in mediating the action of meta-diamides.. In this study, to confirm this result at the organismal level, we employed CRISPR/Cas9-mediated genome editing, generated six transgenic Drosophila strains carrying substitutions in these amino acid residues, and investigated their sensitivity to broflanilide and isocycloseram.

    Article Title: A missense mutation in human INSC causes peripheral neuropathy
    Article Snippet: .. The gRNA for CRISPR/Cas9 target sites was designed using UCSC genome browser ( https://genome.ucsc.edu/ ), and ligated into pBFv-U6.2 (Addgene #138400) plasmid. .. To clone the donor DNA plasmid, both upstream and downstream homology arms of dInsc were amplified from fly genomic DNA by Phusion® High-Fidelity DNA Polymerase (New England Biolab #M0530S) under optimized conditions.

    Expressing:

    Article Title: The scaffolding protein Cnk binds to the receptor tyrosine kinase Alk to promote visceral founder cell specification in Drosophila .
    Article Snippet: .. Target sequences were cloned into the pBFv-U6.2 or pU6-BbsI-chiRNA gRNA expression vectors (GEPC, respectively; Addgene). .. To achieve molecularly defined deletions, we forced homologous recombination by simultaneously injecting (BestGene Inc.) sgRNA(s) and the respective pBluescript gene editing donor construct into embryos carrying the P{FRT(whs)}G13 insertion and expressing Cas9 in their germ line (M{vas-Cas9}ZH-2A).



    Similar Products

    93
    Addgene inc pbfv u6 2 vector
    Pbfv U6 2 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbfv+u6+2/pBFv-U6%2E2+(Plasmid+%23138400)/bio_rxiv__2025__04__25__650632-170-31-33
    Average 93 stars, based on 1 article reviews
    pbfv u6 2 vector - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Addgene inc pbfv u6 2
    Pbfv U6 2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbfv+u6+2/pBFv-U6%2E2+(Plasmid+%23138400)/pmc11099080-271-18-19
    Average 93 stars, based on 1 article reviews
    pbfv u6 2 - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Addgene inc ha tag fused to c terminus pbfv u6 2
    Ha Tag Fused To C Terminus Pbfv U6 2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbfv+u6+2/pBFv-U6%2E2+(Plasmid+%23138400)/pmc11099080__44321_2024_62_MOESM1_ESM-86-80-87
    Average 93 stars, based on 1 article reviews
    ha tag fused to c terminus pbfv u6 2 - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Addgene inc ucsc genome browser
    Ucsc Genome Browser, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbfv+u6+2/pBFv-U6%2E2+(Plasmid+%23138400)/pm38589651-221-9-17
    Average 93 stars, based on 1 article reviews
    ucsc genome browser - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Addgene inc pscarlesshd dsred addgene
    Pscarlesshd Dsred Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbfv+u6+2/pBFv-U6%2E2+(Plasmid+%23138400)/pmc11099080__44321_2024_62_MOESM1_ESM-86-89-90
    Average 93 stars, based on 1 article reviews
    pscarlesshd dsred addgene - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Addgene inc u6 promoter plasmid vector
    U6 Promoter Plasmid Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbfv+u6+2/pBFv-U6%2E2+(Plasmid+%23138400)/pm38458683-57-11-16
    Average 93 stars, based on 1 article reviews
    u6 promoter plasmid vector - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    Image Search Results